The UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) is compatible with the following library prep kits:
QuantSeq 3’ mRNA-Seq V2 Library Prep Kit FWD with UDI (Cat. No. 191 - 196)
QuantSeq 3' mRNA-Seq Library Prep Kits for Illumina (FWD, Cat. No. 015, 113 – 115, 129 – 131)
QuantSeq libraries produced with the kits listed above when used in combination with the QuantSeq-Flex First Strand Synthesis Module for Illumina V2 (Cat. No. 166)
These modules can also be used for QuantSeq library preparation where UMIs are included:
Globin Block Modules for QuantSeq (Human and Pig, Cat. No. 070, 071)
i5 Dual Indexing Add-on Kits for QuantSeq / CORALL (Cat. No. 047)
Lexogen UDI 12 nt Unique Dual Indexing V2 Add-on Kits (Cat. No. 198 - 203) and the V1 of the Lexogen UDI 12 nt Unique Dual Indexing Add-on Kits (Cat. No. 107 - 111, and 120)
UDI 12 nt Unique Dual Indexing Sets (Cat. No. 101 - 105, and 156)
PCR Add-on Kit for Illumina (Cat. No. 020)
This UMI module is not compatible with the following kits:
QuantSeq 3' mRNA-Seq Library Prep Kit REV (for Illumina, Cat. No. 016)
QuantSeq-Flex Second Strand Synthesis Module for Illumina (Cat. No. 028)
CORALL V2 Library Prep Kits (Cat. No. 177 - 182 for CORALL mRNA-Seq V2 and Cat. No. 171 – 176, 183 - 186 for CORALL V2 Total RNA-Seq) and the V1 of these kits (Cat. No. 158 – 163 for CORALL mRNA-Seq and Cat. No. 095, 096, 117 – 119, 132 – 134, 146, 147 for CORALL Total RNA-Seq)
Small RNA-Seq Library Prep Kit for Illumina (Cat. No. 052, 058, 135)
ATTENTION! The UMI Second Strand Synthesis Module for QuantSeq is also not compatible with, and not recommended for use with library prep protocols or kits from any other vendors.