What is the difference between the PCR Add-on Kit (Cat. No. 020) and the PCR Add-on and Reamplification Kit V2 (Cat. No. 208)?

The new PCR Add-on and Reamplification Kit V2 (Cat. No. 208) includes a new and improved PCR system and combines the application purposes functions of the PCR Add-on Kit (Cat. No. 020) and the Reamplification Add-on Kit (Cat. No. 080) in one single kit.

In the V2 Kit, the PCR Mix “PM” and the PCR enzyme Mix “PE” replace the PCR Mix “PCR” and the Enzyme Mix “E” provided with of the PCR Add-on Kit. Additionally, both primers used for qPCR (“P7” and “P5”) are now provided separately (they are not included in the PCR Mix), allowing for a universal use of the kit. For library reamplification, the V2 Reamplification primer Mix “RE” replaces the i7 reamplification Primer “i7-RE” included in the V1 Kit.

NOTE: With the updated PCR system, a new PCR program has also been implemented. Please refer to the respective User Guide p6 for the qPCR assay and p12 for the library reamplification for specific details.

IMPORTANT: For the qPCR assay, the new PCR Add-on and Reamplification Kit V2 is not recommended in combination with older versions (V1) of QuantSeq and CORALL (see table below). When using libraries prepared with previous versions, please refer to the PCR Add-on Kit (Cat. No. 020). In combination with these kits, please use the PCR Add-on Kit (Cat. No. 020).

Compatibility information

Compatibility of the PCR Add-on and Reamplification Kit V2 (Cat. No. 208) with QuantSeq and CORALL V1 and V2.

Compatibility of PCR Add-on Kit V1 (Cat. No. 020) with QuantSeq and CORALL V1 and V2.

*For these kits, the PCR Add-on and Reamplification Kit V2 (Cat. No. 208) is the optimal choice for qPCR and library reamplification.