Libraries prepared with the QuantSeq 3' mRNA-Seq REV require a Custom Sequencing Primer Version 5 (CSPV5), for Read 1 sequencing. Therefore, REV libraries can be sequenced on all illumina platforms that support the use of custom sequencing primers for read 1.
These platforms include: NextSeq 500 / 550, NextSeq 2000, HiSeq 2000 / 2500 / 3000 / 4000, NovaSeq 6000, and MiSeq instruments.
QuantSeq REV libraries are not compatible with the iSeq 100 instrument, as this platform does not support the use of custom sequencing primers.
ATTENTION! Always consult the Illumina Custom Sequencing Primer guide for the relevant instrument.
NOTE: Lexogen does not have direct experience with all of these platforms and reagent versions. These recommendations are based on custom sequencing primer compatibility for these systems. Please inquire at support@lexogen.com for updates, or contact illumina technical support for further details of custom primer use for your specific sequencing setup.
Please also check loading amount guidelines for QuantSeq REV libraries here Do you have recommended loading concentrations for Lexogen libraries on Illumina sequencing instruments? . In general we recommend loading QuantSeq REV libraries at the higher range of Illumina-provided specifications.