Processing 1 µg of high-quality Arabidopsis thaliana RNA with RiboCop for Plants typically results in ~3% remaining rRNA reads, while processing 1-100 ng results in ~1% remaining rRNA reads.
Depletion rates may vary depending on the quality of the RNA and purity of the sample. Contaminants, such as salts, metal ions, and organic solvents, may have a negative impact on the efficiency of the protocol