What does a typical LUTHOR HD Pool library look like?

With LUTHOR HD Pool, the majority of inserts are greater than 100 bp in size, corresponding to final library fragment sizes ≥300 bp.

IMPORTANT! Library yield, shape, and average insert size may vary, depending on the type of input sample (e.g., degraded, low quality RNA produces shorter library inserts than high quality RNA). Additionally, cell culture medium can interfere with and negatively impact library preparation. When using cells in culture media, if possible, wash with PBS before dispensing into LUTHOR Cell Lysis Buffer (CLB). For more information, please contact support@lexogen.com.