What are the cell sample concentration requirements?

Cells should be in a single-cell suspension in any conductive cell culture medium or buffer.

The ideal input is a minimum of 150,000 cells in at least 1ml cell culture medium or buffer.

1ml is the minimal volume required to use with the cell strainer.

IMPORTANT: When using lower volumes (i.e., <1ml), it is not possible to use the cell strainer to filter the cell suspension. In this case, please contact technical support at support@lexogen.com for further recommendations.

Once the cells are filtered, please adjust the cell concentration to 1.5.10^{^}5 cells/ml. Then 15µl will be used for the sample preparation with the LUTHOR scd buffer for a final working concentration of 15cells/µl (15.000 cells/ml).

IMPORTANT: Lower cell numbers can also be used (down to 100 cells). Please contact technical support at support@lexogen.com for further information.

NOTE: If cells tend to aggregate, it is possible to optimize the medium using serum-free, Ca²⁺/Mg²⁺- free medium, or by adding chelators, DNase, or anti-clumping compounds.