Lexogen Online FAQs

What loading amount is recommended for QuantSeq FWD-UMI libraries?

Owned by Matteo Carina (Unlicensed)
Last updated: Aug 05, 2024 by Nathalie Durut
1 min read

Lane mix and PhiX concentrations required for sequencing QuantSeq FWD-UMI libraries differ from those used for standard QuantSeq FWD libraries. This is related to the presence of the low-diversity 4 nt spacer immediately after the 6 nt UMI sequence. This low diversity spacer can impact read 1 quality over cycles 7 - 10 of the run. For this reason 5 – 30 % of PhiX should be spiked into all QuantSeq FWD lanemixes.

Optimal PhiX spike-in percentages also vary between instruments.

For full details please see the updated table in: Do you have recommended loading concentrations for Lexogen libraries on Illumina sequencing instruments?




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