How can I QC my RNA after using RiboCop for Yeast?

As the rRNA in yeast is extremely high (~97% of total RNA), microfluidics-based platforms such as Bioanalyzer and TapeStation may not be sensitive enough to detect the output from RiboCop and thus are not recommended for QC. We would recommend to insert RNA directly into the intended downstream application i.e., library preparation kit.

If you must QC RNA after RiboCop, an RT-qPCR or RT-PCR assay can be done with rRNA-specific primers to assess rRNA abundance pre- and post-depletion e.g., using a one-step RT qPCR kit together with primers for yeast 26S rRNA.