How is genomic DNA removed with the SPLIT RNA Extraction Kit?

The SPLIT protocol efficiently removes genomic DNA from most cell types with the help of acidic phenol. However, certain sample types, such as blood, contain unusually high proportions of DNA in comparison to RNA. Due to this imbalance, gDNA carry over may occur. This can be seen as a well-retarded band in agarose gel runs and on microfluidic devices such as the Fragment Analyzer in setups that allow for simultaneous analysis of RNA and high-molecular weight DNA. For these difficult sample types, DNase treatment may be required prior to processing of the extracted RNA. Additionally, DNase treatment is also recommended when RNA will be used downstream as input for sensitive applications such as RNA-Seq or qRT-PCR.

DNA removal can be performed using our Lexogen´s DNA Removal Add-on module (Cat. No. 235).

For more information, please contact support@lexogen.com where we can support you with protocol suggestions tailored to your workflow.