The UMI module can also be used for preparing QuantSeq-Flex libraries using targeted priming for first strand cDNA synthesis and , as long as random priming is used for second strand synthesis.
For use with QuantSeq-Flex, the UMI Second Strand Synthesis Mix (USS) also replaces the Second Strand Synthesis Mix 1 (SS1) from the standard QuantSeq kit at step 7 of the protocol for Random Primed Second Strand Synthesis.