| Table of Contents | ||||
|---|---|---|---|---|
|
...
ATTENTION! The guidelines outlined below do not apply for QuantSeq-Pool or LUTHOR 3' mRNA-Seq library preparation protocols.
Instead, please consult the QuantSeq-Pool User Guide, Appendix B, and this online FAQ, and the LUTHOR 3' mRNA-Seq User Guide.
...
The PCR Add-on Kit for Illumina (Cat. No. 020.96), and
SYBR Green I nucleic acid stain (10,000X in DMSO, not provided in the kit).
ATTENTION: ! The use of SYBR Green I-containing qPCR mastermixes from other vendors is not recommended.
Prepare SYBR Green I 2.5x Working Stock
...