Sample FFPE tissue sample input should be 100-800mm800 mm2 in surface or 1-15mg FFPE tissue15 mg in weight, and up to 10µm 10 µm in thickness. If For higher input amounts are used, we cannot guarantee results and would recommend to first perform strongly recommend performing a pilot experiment first to evaluate RNA extraction efficiency. If you have questions, please contact support@lexogen.com.
Below are general recommendations for maximum input, depending on the thickness and surface area of the FFPE slices:
Slice thickness | Maximum input |
5 µm | 1 slice with 1,600 mm2 2 slices with 800 mm2 3 slices with 530 mm2 4 slices with 400 mm2 |
10 µm | 1 slice with 800 mm2 2 slices with 400 mm2 3 slices with 260 mm2 |
20 µm | 1 slice with 400 mm2 2 slices with 200 mm2 3 slices with 130 mm2 4 slices with 100 mm2 |
For lower inputs, please refer to the following FAQ: How can I increase my final yield?