| Title: | TeloPrime | |
|---|---|---|
| Owner: | Matteo Carina | |
| Creator: | Matteo Carina | Jun 18, 2021 |
| Last Changed by: | Simona Ferraioli | Nov 23, 2023 |
| Tiny Link: (useful for email) | https://lexogen-customerportal.atlassian.net/wiki/x/dYDLAw | |
| Export As: | Word · PDF | |
Hierarchy
Children (17)
What are the input RNA requirements for TeloPrime?
Can I use low quality RNA as input for TeloPrime Full-Length cDNA Amplification?
How are the 5’ and 3’ ends tagged?
How long does the generation of full-length double-stranded DNA take?
Can I use custom primers for reverse transcription or PCR?
Why do I need to perform a qPCR? Can this step be skipped?
How is the cycle number of the endpoint PCR determined?
How can I check the optimal PCR cycle number if I cannot perform the qPCR assay?
Which sequences are used for PCR amplification?
How Does TeloPrime V2 perform for different types of RNA?
What are the input RNA requirements for TeloPrime?
Can I use low quality RNA as input for TeloPrime Full-Length cDNA Amplification?
How are the 5’ and 3’ ends tagged?
How long does the generation of full-length double-stranded DNA take?
Can I use custom primers for reverse transcription or PCR?
Why do I need to perform a qPCR? Can this step be skipped?
How is the cycle number of the endpoint PCR determined?
How can I check the optimal PCR cycle number if I cannot perform the qPCR assay?
Which sequences are used for PCR amplification?
How Does TeloPrime V2 perform for different types of RNA?
