| Title: | SLAMseq | |
|---|---|---|
| Owner: | Matteo Carina | |
| Creator: | Matteo Carina | Jun 18, 2021 |
| Last Changed by: | Lauren Sheehan | Jan 16, 2024 |
| Tiny Link: (useful for email) | https://lexogen-customerportal.atlassian.net/wiki/x/ogDOAw | |
| Export As: | Word · PDF | |
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Children (15)
Do I need to get all 4 modules or can I just get the kinetics kit(s)?
How long should the labeling time be?
What is the shortest labeling duration recommended before beginning time course sampling?
Do you have guidelines for the optimal S4U concentrations to use for different cell lines?
Do I need to change S4U-containing medium every 3 hours?
What solution is the 4-Thiouridine (S4U) dissolved in?
Which reverse transcriptase should be used to read through alkylated S4U nucleotides?
Are there some additional instructions for how to run the HPLC analysis for the S4U Incorporation assay (e.g. Column, temperature, injection volume, and flow rate)?
How do I calculate the S4U Incorporation Rate?
Should I treat my SLAMseq RNA samples with DNase I and should this be done before or after alkylation with iodoacetamide?
Do I need to get all 4 modules or can I just get the kinetics kit(s)?
How long should the labeling time be?
What is the shortest labeling duration recommended before beginning time course sampling?
Do you have guidelines for the optimal S4U concentrations to use for different cell lines?
Do I need to change S4U-containing medium every 3 hours?
What solution is the 4-Thiouridine (S4U) dissolved in?
Which reverse transcriptase should be used to read through alkylated S4U nucleotides?
Are there some additional instructions for how to run the HPLC analysis for the S4U Incorporation assay (e.g. Column, temperature, injection volume, and flow rate)?
How do I calculate the S4U Incorporation Rate?
Should I treat my SLAMseq RNA samples with DNase I and should this be done before or after alkylation with iodoacetamide?
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