Do you have guidelines for the optimal S4U concentrations to use for different cell lines?

The following table provides S4U concentrations and IC_10ti values (optimal labeling concentrations) that have been evaluated for various cell lines. These concentrations are given as guidelines only; further optimization could be required. We recommend verifying optimal labeling concentrations using the SLAMseq Explorer Kit – Cell Viability Titration Module (Cat. No. 059) when establishing SLAMseq.

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Additionally, as a starting point for planning your SLAMseq experiments we recommend a literature search to determine whether S4U concentrations for your specific cell type have been characterized before. Some references that have reviewed S4U concentrations are given below:

• Russo, J., Heck, A. M., Wilusz, J., & Wilusz, C. J. (2017). Metabolic labeling and recovery of nascent RNA to accurately quantify mRNA stability. Methods, 120, 39-48.

• Duffy, E. E., Schofield, J. A., & Simon, M. D. (2019). Gaining insight into transcriptome‐wide RNA population dynamics through the chemistry of 4‐thiouridine. Wiley Interdisciplinary Reviews: RNA, 10(1), e1513.