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How does TraPR achieve species-independent isolation of functional sRNAs?
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How does TraPR achieve species-independent isolation of functional sRNAs?
- Stephanie Bannister
- Lauren Sheehan
- Matteo Carina (Unlicensed)
Owned by Stephanie Bannister
TraPR relies on the high conservation of structural and biochemical properties of AGO proteins to specifically select sRNA-associated AGO ribonucleoprotein complexes.
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Is TraPR-isolated sRNA suitable for high-throughput sequencing?
Is TraPR-isolated sRNA suitable for high-throughput sequencing?
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Can I extract the RNA downstream of TraPR with methods other than phenol-chloroform, e.g. with TRIzol or silica spin-columns?
Can I extract the RNA downstream of TraPR with methods other than phenol-chloroform, e.g. with TRIzol or silica spin-columns?
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Do I need any extra reagents to purify the sRNAs?
Do I need any extra reagents to purify the sRNAs?
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