What are the concentrations of the SIRVs?

SIRV-Set 1 contains three isoform mixes, and while the concentration of most individual SIRV isoforms is different in Mixes E0, E1, and E2, they each have the a total concentration of 25.3 ng/µa and a total molarity of about 70 fmol/µl (69.0, 68.5 and 70.8 fmol/µl). In Mix E0, all SIRVs are present at the same molarity.

SIRV-Set 2 contains the Iso Mix E0 with 69 SIRV isoforms at 1.0 fmol each. The total amount of RNA is 69.0 fmol corresponding to 25.2 ng.

SIRV-Set 3 combines the equimolar mix of the 69 SIRV isoforms (Iso Mix E0) at 0.6 fmol each and 92 single-isoform transcripts (ERCCs) ranging from 0.014 amol to 15.0 fmol. The total amount of RNA is 93.2 fmol or 30.3 ng per tube.

SIRV-Set 4 combines the equimolar mix of the 69 SIRV isoforms (Iso Mix E0) at 0.6 fmol each, the 92 single-isoform transcripts (ERCCs) ranging from 0.014 amol to 15.0 fmol, and the equimolar mix of 15 long SIRVs at 0.6 fmol each. The total amount of RNA is 102.2 fmol or 53.5 ng per tube.

Table 1: Compositions of SIRV-Set 2, SIRV-Set 3, and SIRV-Set 4