Lexogen Online FAQs

How to use SIRVs

Owned by Matteo Carina (Unlicensed)
Last updated: Nov 07, 2021 by Stephanie Bannister
1 min read

SIRV RNA is spiked into samples prior to RNA Extraction, or into total RNA prior to pre-processing or library preparation. The amount of SIRV RNA to add to each sample depends on three things:

  • the total RNA input amount,

  • type of library preparation method used, and

  • the intended fraction of SIRV reads to be detected in the final sequencing output.

 

Use our Updated SIRV Calculator Worksheets to plan your spike-in experiments!

 

Download the User Guides and Reference Annotations from our SIRV Download page.

Access the updated SIRV Suite Data Analysis Tools.

Have feedback for us or need more information? Send us a request or write to us at support@lexogen.com.