How to use SIRVs

SIRV RNA is spiked into samples prior to RNA Extraction, or into total RNA prior to pre-processing or library preparation. The amount of SIRV RNA to add to each sample depends on three things:

• the total RNA input amount,

• type of library preparation method used, and

• the intended fraction of SIRV reads to be detected in the final sequencing output.

Use our Updated SIRV Calculator Worksheets to plan your spike-in experiments!

• SIRV-Set 1 Spike-in Calculator

• SIRV-Set 2 Spike-in Calculator

• SIRV-Set 3 Spike-in Calculator

• SIRV-Set 4 Spike-in Calculator

Download the User Guides and Reference Annotations from our SIRV Download page.

Access the updated SIRV Suite Data Analysis Tools.