Which RNAs are isolated using the Poly(A) RNA Selection Kit?

The Poly(A) RNA Selection Kit enriches for all RNAs that have a poly(A) tail, such as mRNA, but also mitochondrial rRNA, which can make up to 4 % of the isolated poly(A) RNA (0.08 % of the total RNA). Therefore, between 2 – 4 % of sequencing reads can correspond to rRNA when using various standard RNA-Seq library preps.