The UMI module can also be used for preparing QuantSeq-Flex libraries using targeted priming for first strand cDNA synthesis and random priming for second strand synthesis. For use with QuantSeq-Flex, the UMI Second Strand Synthesis Mix (USS) also replaces the Second Strand Synthesis Mix 1 (SS1) from the standard kit at step 7 of the protocol for Random Primed Second Strand Synthesis.
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