FFPE tissue sample input should be 100-800 mm2 in surface or 1-15 mg in weight, and up to 10 µm in thickness. For higher input amounts, we strongly recommend performing a pilot experiment first to evaluate RNA extraction efficiency.
Below are general recommendations for maximum input, depending on the thickness and surface area of the FFPE slices:
Slice thickness | Maximum input |
5 µm | 1 slice with 1,600 mm2 2 slices with 800 mm2 3 slices with 530 mm2 4 slices with 400 mm2 |
10 µm | 1 slice with 800 mm2 2 slices with 400 mm2 3 slices with 260 mm2 |
20 µm | 1 slice with 400 mm2 2 slices with 200 mm2 3 slices with 130 mm2 4 slices with 100 mm2 |
For lower inputs, please refer to the following FAQ: How can I increase my final yield?