Is the kit suitable for preparation of libraries from degraded RNA or FFPE samples?

Formalin-Fixed, Paraffin-Embedded (FFPE) material is very heterogeneous and highly variable in terms of quality, degree of cross-linking, and accessibility of the mRNA. Therefore, processing degraded or FFPE RNA with QuantSeq-Pool may lead to uneven read distribution between samples in sequencing experiments.

For best practice, the QuantSeq FWD 3' mRNA-Seq V2 Library Prep Kit with UDIs (Cat. No. 191 - 196) is recommended for processing FFPE samples, as it allows quality control and quantification of individual libraries.

This offers the possibility to re-adjust individual samples for equimolar lane pooling and balanced read distribution during sequencing.