What input amounts of RNA can be used?

The recommended input range for efficient depletion with RiboCop rRNA Depletion Kits for Bacteria is 1 – 1,000 ng of total RNA for monocultures of Gram Negative or Gram Positive bacteria using the respective G- and G+ Probe Mixes (Cat. No. 126 and 127, respectively). We suggest initial experiments to be performed using 100 ng of input material. The META Probe Mix (Cat. No. 125) is primarily suited for depletion of 1 – 1,000 ng of total RNA from mixed bacterial samples. The META Probe Mix may also be used at 1 – 100 ng when working with monocultures.