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Should I perform a DNase treatment before QuantSeq FFPE?
Lexogen Online FAQs
Should I perform a DNase treatment before QuantSeq FFPE?
- Lauren Sheehan
- Nathalie Durut (Unlicensed)
- Branislav.Lilic
Owned by Lauren Sheehan
As the risk of gDNA contamination is higher due to the degradation of RNA in FFPE samples, it is recommended to perform a DNase treatment prior to library prep.
Two options for DNase treatment of FFPE samples:
DNA Removal Add-on Module (Cat. No. 235) or
SPLIT One-Step FFPE RNA Extraction Kit (Cat. No. 236)
, multiple selections available,
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