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Are Unique Molecular Identifiers (UMIs) included in QuantSeq FFPE?
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Are Unique Molecular Identifiers (UMIs) included in QuantSeq FFPE?
Yes, a 12nt UMI sequence is included in the oligo(dT) primer and, therefore, located at the beginning of Read 2.
To read out the UMI sequence, a paired-end sequencing run is required (for more information, please see the following FAQs: Can I use single read sequencing for QuantSeq FFPE? and What sequencing read length and read depth are recommended for QuantSeq FFPE?.
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