Lexogen Online FAQs

Are Unique Molecular Identifiers (UMIs) included in QuantSeq FFPE?

Yes, a 12nt UMI sequence is included in the oligo(dT) primer and, therefore, located at the beginning of Read 2.

To read out the UMI sequence, a paired-end sequencing run is required (for more information, please see the following FAQs: Can I use single read sequencing for QuantSeq FFPE? and What sequencing read length and read depth are recommended for QuantSeq FFPE?.

Have feedback for us or need more information? Send us a request or write to us at support@lexogen.com.