/
Are Unique Molecular Identifiers (UMIs) included in QuantSeq FFPE?

Lexogen Online FAQs

Are Unique Molecular Identifiers (UMIs) included in QuantSeq FFPE?

Yes, a 12nt UMI sequence is included in the oligo(dT) primer and, therefore, located at the beginning of Read 2.

To read out the UMI sequence, a paired-end sequencing run is required (for more information, please see the following FAQs: Can I use single read sequencing for QuantSeq FFPE? and What sequencing read length and read depth are recommended for QuantSeq FFPE?.

Related content

Have feedback for us or need more information? Send us a request or write to us at support@lexogen.com.