How can I use SIRVs to measure the mRNA content of my sample?

For samples with unknown mRNA content we recommend to assume a 3% mRNA content, and spike in SIRVs to target 1% of final mapped reads. By comparing the share of reads aligning to the reference genome and the "SIRVome", respectively you can then derive the mRNA content.

For further details, please consult the SIRV User Guides (https://www.lexogen.com/docs/sirvs-mixes/ ).