Lexogen Online FAQs
How many samples can I spike-in with the SIRV mixes?
- Matteo Carina (Unlicensed)
- Stephanie Bannister
Owned by Matteo Carina (Unlicensed)
The number of reactions depends on the spike-in amount required. You can estimate the number of samples possible to spike in using the respective SIRV-Set Spike-In Calculator Worksheets:
From the SIRV-Set 1 mixes you can draw 4 times 1 µl. This 1 µl should then be stepwise diluted to the optimal working concentration.
From SIRV-Set 2, 3, and 4 mixes you can draw 9 times 1 µl, and similar dilutions should be made.
The optimal working dilution will depend on the amount of SIRV RNA to spike-into each sample as well as the volume of SIRVs that can be added to each sample. See also Equation 2 in the SIRV User Guides.
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