Can I store SIRV dilutions for further use?

No. SIRV dilutions should be considered as single-use only and should not be freeze-thawed.

We do not recommend storing SIRV dilutions as the risk of degradation and adsorption to the tube increases over time, even with optimal storage at or below -20 degrees Celsius.

For this reason we recommend to aliquot the SIRV stock solutions upon first use into 1 ul volumes, and store at or below -20 degrees until needed.

Best practice is to prepare and use SIRV dilutions immediately before use, keeping the stock and dilutions continuously on ice, and using careful RNase-free handling technique, to minimize the risk of degradation.

Please see the SIRV User Guides for specific guidelines for handling SIRV dilutions.